Zoekresultaten - 8 results
Strategy for the identification of micro-organisms producing food and feed products: Bacteria producing food enzymes as study case
Second, an in-house database, with 16S reference sequences from bacteria producing FE, was constructed for their fast identification through blast analysis. Third, the sensitivity was assessed on a spiked ...
Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.
Besides the technological advances which have pushed the approach forward, the last years have been marked by considerable evolution of the whole genome sequencing data analysis methods. Prior to ...
An integrated strategy combining DNA walking and NGS to detect GMO
Volume 232, Number 358 (2017) Keywords: detection DNA walking GMO Next-generation sequencing qPCR Abstract: Recently, we developed a DNA walking system for the detection and characterization of a broad ...
Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.
Keersmaecker; Katelijne Dierick; Sophie Bertrand Source: J Antimicrob Chemother, Volume 71, Issue 9, p.2479-83 (2016) Keywords: Bacteriological Techniques beta-Lactamases Cost-Benefit Analysis Genotyping ...
Strategies to detect unauthorized GMO in the food and feed chain (PhD thesis)
https://www.sciensano.be/nl/biblio/strategies-detect-unauthorized-gmo-food-and-feed-chain-phd-thesis
pCAMBIA), were selected to develop a strategy allowing to detect and characterize a broad range of GMO. This strategy, fully integrated in the GMO routine analysis, consists to characterize sequences ...
Data analysis associated with the use of whole genome sequencing for subtyping of pathogenic microorganisms, Salmonella Typhimurium as a case study
Bioinformatics in Life Sciences, NA, Issue VIB, NA (2016) Keywords: a an analysi analysis application approach approaches AS at bioinformatics Case case studies Case study case-study Comparison continue Cost data ...
Current and new approaches in GMO detection: challenges and solutions
https://www.sciensano.be/nl/biblio/current-and-new-approaches-gmo-detection-challenges-and-solutions
DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR) is the method of choice for the enforcement laboratories in GMO routine analysis ...
Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing
pioneers already investigated Next Generation Sequencing (NGS) as an alternative to qPCR, its practical use has not been assessed for routine analysis. In this study a statistical framework was developed to ...