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PCR detects HCV RNA in a plasma pool contaminated by a single preseroconversion donation of genotype 5a.

Specificity Serologic Tests Viral Load Abstract: BACKGROUND AND OBJECTIVES: To determine the prevalence of HCV- RNA-positive plasma pools in Belgium, to validate our PCR method and to increase the safety of the ...

Prevalence of bovine herpesvirus-1 in the Belgian cattle population.

Allocation Seroepidemiologic Studies Vaccination Abstract: The national bovine herpesvirus 1 (BHV-1) seroprevalence (apparent prevalence) in the Belgian cattle population was determined by a serological survey ...

Production of antibodies against chicken interferon-gamma: demonstration of neutralizing activity and development of a quantitative ELISA.

Blot analysis or natural ChIFN-gamma in immunofluorescence experiments. As determined by competition ELISA s, mAbs 3D5, 4C6 and 3A3 recognized the same or adjacent epitopes on the ChIFN-gamma molecule, ...

Trends in gonorrhoea in nine western European countries, 1991-6. European Study Group.

indicate that in more recent years there was an increase in the number of gonorrhoea cases among men having sex with men in some countries. Further investigations are necessary to determine if this ...

Nosocomial pseudo-outbreak of Fusarium verticillioides associated with sterile plastic containers3234010

Examination of clinical records and handling procedures revealed a fungal contamination of supposedly sterile containers used for biological materials. An accurate system of monitoring permitted us to determine ...

Comparison of biological activities of natural and recombinant chicken interferon-gamma.

replication of cytolytic viruses in chicken embryo fibroblasts and to activate macrophages, as was determined by nitric oxide production. Levels ranging between 100 and 300 microg/ml of BacChIFN-gamma could be ...

Phylogenetic analysis of European encephalomyocarditis viruses: comparison of two genomic regions.

The phylogenetic relationships of encephalomyocarditis (EMC) viruses isolated from pigs and rodents in Europe were determined by comparison of nucleotide sequences from two different regions of the ...

Immunogenicity and protective efficacy of DNA vaccines encoding secreted and non-secreted forms of Mycobacterium tuberculosis Ag85A.

DNA Abstract: OBJECTIVE: To determine the efficacy of Ag85A- DNA against challenge with a highly virulent human clinical isolate of Mycobacterium tuberculosis (CSU37) and to compare the potencies of two ...

Comparison of eae, tir, espA and espB genes of bovine and human attaching and effacing Escherichia coli by multiplex polymerase chain reaction44

The sequences alignments revealed the presence of constant and variable regions. Multiplex polymerase chain reactions were developed, in order to determine the subtype of each gene present in ...

Detection and genotyping of bovine diarrhea virus by reverse transcription-polymerase chain amplification of the 5' untranslated region.

bovine diarrhea virus (BVDV) from other pestiviruses, and to determine the genotype of the BVDV isolates. For this purpose, primer pairs were selected in the 5’ untranslated region (5’ UTR). The primers BE ...

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