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Retrospective evaluation of routine whole genome sequencing of Mycobacterium tuberculosis at the Belgian National Reference Center, 2019.
switched from conventional typing to prospective whole-genome sequencing (WGS) of all submitted complex (MTB) isolates. The ISO17025 validated procedure starts with semi-automated extraction and purification ...
Identification of Molds with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Performance of the Newly Developed MSI-2 Application in Comparison with the Bruker Filamentous Fungi Database and MSI-1.
satisfactory results when identifying cryptic species. Here, we aimed to validate a new web application, MSI-2, which replaces MSI-1, an application that was built and deployed online in 2017. For the ...
Surveillance de la mortalité COVID-19 en Belgique, épidémiologie et méthodologie durant la 1re et 2e vague (mars 2020- 14 février 2021)
fortement corrélée avec la surmortalité toutes causes confondues en Belgique. La surmortalité a été un indicateur clé dans l’épidémie de COVID-19 pour valider le fait que la déclaration épidémiologique de la ...
Surveillance of COVID-19 mortality in Belgium, epidemiology and methodology during 1st and 2nd wave (March 2020- 14 February 2021)
correlated with excess all-cause mortality in Belgium. The excess mortality was a key indicator in the COVID-19 epidemic to validate that the epidemiological reporting of COVID-19-related mortality was ...
Occurrence of (suspected) genotoxic flavoring substances in Belgian alcohol-free beers
evaporation (SAFE) technique coupled to GC – MS (SIM) was developed and validated. Focus was put on authorized flavoring substances requiring specific attention due to a genotoxic concern based on information ...
A Targeted UHPLC-MS/MS Method Validated for the Quantification of Ergot Alkaloids in Cereal-Based Baby Food from the Belgian Market
QuEChERS UHPLC-MS/MS VALIDATION Abstract: Following pending new legislation in the European Union setting a maximum of 20 ng g−1 for the total sum of ergot alkaloids in dry cereal-based baby food, a new ...
Verification of a non-validated reference method according to ISO 16140-3: an example: Detection of botulinum neurotoxin producing Clostridia following ISO/TS 17919
Publication Type: Scientific poster, presentation or proceeding Authors: Tom Van Nieuwenhuysen Source: BSFM 25th conference on Food Microbiology (2021) Keywords: C. botulinum ISO 16140 Verification Health Topics: Botulisme Botulism Botulisme Service: Pa ...
Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking
real-time PCR GMM protease2 marker was also demonstrated on microbial fermentation products. To confirm its use by other GMO enforcement laboratories, the transferability of the in-house validated real-time ...
Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV.
able to distinguish Neethling-based LAV s and wild-type LSDV were developed. Upon validation, both assays were shown to have high sensitivity and specificity with a diagnostic performance comparable to ...
In-vivo expressed Mycobacterium tuberculosis antigens recognised in three mouse strains after infection and BCG vaccination.
potential for boosting. Thus, recognition of promising Mtb-antigens identified in humans validates across multiple mouse TB-infection models with widely differing TB-susceptibilities. This offers ...