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2025
Assessing the authenticity and purity of a commercial Bacillus thuringiensis bioinsecticide through whole genome sequencing and metagenomics approaches, Yari, Van Laere, Fraiture Marie-Alice, Gobbo Andrea, De Keersmaecker Sigrid C. J., Marchal Kathleen, Roosens Nancy, and Vanneste Kevin , Frontiers in Microbiology, Jul-01-2027, Volume 16, (2025)
Galaxy @Sciensano: a comprehensive bioinformatics portal for genomics-based microbial typing, characterization, and outbreak detection., Bogaerts, Bert, Van Braekel Julien, Van Uffelen Alexander, D'aes Jolien, Godfroid Maxime, Delcourt Thomas, Kelchtermans Michael, Milis Kato, Goeders Nathalie, De Keersmaecker Sigrid C. J., et al. , BMC Genomics, 2025 Jan 08, Volume 26, Issue 1, (2025)
Metagenomics-based tracing of genetically modified microorganism contaminations in commercial fermentation products, D'aes, Jolien, Fraiture Marie-Alice, Bogaerts Bert, Van Laere Yari, De Keersmaecker Sigrid C. J., Roosens Nancy, and Vanneste Kevin , Food Chemistry: Molecular Sciences, June-2025, Volume 10, (2025)
2024
Benchmarking bacterial taxonomic classification using nanopore metagenomics data of several mock communities, Van Uffelen, Alexander, Posadas Andrés, Roosens Nancy, Marchal Kathleen, De Keersmaecker Sigrid C. J., and Vanneste Kevin , Scientific Data, 2024/08/10, Volume 11, Issue 1, (2024)
be.Prepared: An architecture to increase Belgian integration of health data as a way of strengthening preparedness for infectious diseases, Van Laer, Amber, Kelchtermans Michael, Millis Kato, Gori A., Van Cauteren Dieter, Brondeel Ruben, De Keersmaecker Sigrid C. J., Herman Philippe, Roosens Nancy, De Ridder Karin, et al. , The Belgian Presidency of the Council of the European Union, March 2024, (2024)
be.Prepared to increase Belgian integration of health data as a way of strengthening preparedness for infectious diseases, Masset, Heleen, Van Laer Amber, Kelchtermans Michael, Millis Kato, Gori A., Winand Raf, De Keersmaecker Sigrid C. J., Roosens Nancy, Herman Philippe, Brondeel Ruben, et al. , ESCMID Global 2024, 27/04/2024, Barcelona, (2024)
Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens., Bogaerts, Bert , J Clin Microbiol, 2024 Mar 05, (2024)
Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens., Bogaerts, Bert, Van den Bossche An, Verhaegen Bavo, Delbrassinne Laurence, Mattheus Wesley, Nouws Stéphanie, Godfroid Maxime, Hoffman Stefan, Roosens Nancy, De Keersmaecker Sigrid C. J., et al. , J Clin Microbiol, 2024 Mar 05, (2024)
Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens., Bogaerts, Bert , J Clin Microbiol, 2024 Mar 05, (2024)
Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens., Bogaerts, Bert , J Clin Microbiol, 2024 Mar 05, (2024)
Genomic comparison between and and analysis of peptide-based biomarkers for serodiagnosis., Moens, Charlotte, Bogaerts Bert, Lorente-Leal Victor, Vanneste Kevin, De Keersmaecker Sigrid C. J., Roosens Nancy, Mostin Laurent, Fretin David, and Marché Sylvie , Front Vet Sci, 2024, Volume 11, (2024)
An interlaboratory proficiency test using metagenomic sequencing as a diagnostic tool for the detection of RNA viruses in swine fecal material., Liu, Lihong, Mikhayil Hakhverdyan, Wallgren Per, Vanneste Kevin, Qiang Fu, Pierrick Lucas, Blanchard Yannick, de Graaf Miranda, Oude Munnink Bas B., van Boheemen Sander, et al. , Microbiol Spectr, 2024 Oct 03, Volume 12, Issue 10, (2024)
Phage-Mediated Digestive Decolonization in a Gut-On-A-Chip Model: A Tale of Gut-Specific Bacterial Prosperity., Van Nieuwenhuyse, Brieuc, Merabishvili Maya, Goeders Nathalie, Vanneste Kevin, Bogaerts Bert, de Jode Mathieu, Ravau Joachim, Wagemans Jeroen, Belkhir Leïla, and Van der Linden Dimitri , Viruses, 2024 Jun 28, Volume 16, Issue 7, (2024)
Pilot market surveillance of GMM contaminations in alpha-amylase food enzyme products: A detection strategy strengthened by a newly developed qPCR method targeting a GM Bacillus licheniformis producing alpha-amylase, Fraiture, Marie-Alice, Gobbo Andrea, Guillitte Chloé, Marchesi Ugo, Verginelli Daniela, De Greve Joke, D'aes Jolien, Vanneste Kevin, Papazova N., and Roosens Nancy , Food Chemistry: Molecular Sciences, Jan-07-2024, Volume 8, (2024)
Strain-level characterization of foodborne pathogens without culture enrichment for outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling, Buytaers, Florence E., Verhaegen Bavo, Van Nieuwenhuysen Tom, Roosens Nancy, Vanneste Kevin, Marchal Kathleen, and De Keersmaecker Sigrid C. J. , Frontiers in Microbiology, (2024)
Towards facilitated interpretation of shotgun metagenomics long-read sequencing data analyzed with KMA for the detection of bacterial pathogens and their antimicrobial resistance genes, Gand, Mathieu, Indre Navickaite, Bartsch Lee-Julia, Grützke Josephine, Overballe-Petersen Søren, Rasmussen Astrid, Otani Saria, Michelacci Valeria, Rodríguez Matamoros, Bosco, González-Zorn Bruno, et al. , Frontiers in Microbiology, Apr-04-2024, Volume 15, (2024)
2023
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion, Gand, Mathieu, Bloemen Bram, Vanneste Kevin, Roosens Nancy, and De Keersmaecker Sigrid C. J. , BMC Genomics, Jan-12-2023, Volume 24, Issue 1, (2023)
Development of a portable on-site applicable metagenomic data generation workflow for enhanced pathogen and antimicrobial resistance surveillanceAbstract, Bloemen, Bram, Gand Mathieu, Vanneste Kevin, Marchal Kathleen, Roosens Nancy, and De Keersmaecker Sigrid C. J. , Scientific Reports, Jan-12-2023, Volume 13, Issue 1, (2023)
A new open strategy for impurity surveillance of commercial microbial fermentation food products, Fraiture, Marie-Alice, Roosens Nancy, Papazova N., De Keersmaecker Sigrid C. J., Vanneste Kevin, Orval Rik, Van Laere Yari, Debode Frédéric, De Loose Marc, Van Poucke Christof, et al. , Brussels, Belgium, (2023)
Novel threats- State of the art of synthetic biology, T. Johansen, Bjordal, Madslien E.H., Forsman M., Ekström F., Fykse E.M., De Keersmaecker Sigrid C. J., Roosens Nancy, Vanneste Kevin, Fraiture Marie-Alice, Palyi B., et al. , Munich, Germany, (2023)
Retrospective surveillance of viable Bacillus cereus group contaminations in commercial food and feed vitamin B2 products sold on the Belgian market using whole-genome sequencing, Bogaerts, Bert, Fraiture Marie-Alice, Van Nieuwenhuysen Tom, Jacobs Bram, Van Hoorde Koenraad, De Keersmaecker Sigrid C. J., Roosens Nancy, and Vanneste Kevin , Frontiers in Microbiology, Sep-06-2024, Volume 14, (2023)
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling, Buytaers, Florence E., Verhaegen Bavo, Van Nieuwenhuysen Tom, Roosens Nancy, Vanneste Kevin, Marchal Kathleen, and De Keersmaecker Sigrid C. J. , BSFM, 2023-10-13, Brussels, Belgium, (2023)
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms, Fraiture, Marie-Alice, D'aes Jolien, Guiderdoni Emmanuel, Meunier Anne-Cécile, Delcourt Thomas, Hoffman Stefan, Vandermassen Els, De Keersmaecker Sigrid C. J., Vanneste Kevin, and Roosens Nancy , Foods, Jan-02-2023, Volume 12, Issue 3, (2023)
Transforming Shiga toxin-producing surveillance through whole genome sequencing in food safety practices., Nouws, Stéphanie, Verhaegen Bavo, Denayer Sarah, Crombé Florence, Piérard Denis, Bogaerts Bert, Vanneste Kevin, Marchal Kathleen, Roosens Nancy, and De Keersmaecker Sigrid C. J. , Front Microbiol, 2023, Volume 14, (2023)
Using a combination of short and long read sequencing to investigate the diversity in plasmid- and chromosomally encoded extended-spectrum-beta-lactamases (ESBL) in clinical Shigella and Salmonella isolates in Belgium, Berbers, Bas, Vanneste Kevin, Roosens Nancy, Marchal K., Ceyssens Pieter-Jan, and De Keersmaecker Sigrid C. J. , Microbial Genomics, 23/01/2023, Volume 9:000925, Number 23, (2023)

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